Liquid Chromatography of Aflatoxins Including Aflatoxins B2a and G2a
نویسندگان
چکیده
منابع مشابه
Determination of Aflatoxins B1 and B2 in Powdered Milk Using Modified Liquid Chromatography Method
Background & Aims: In this study, using a liquid-liquid microextraction method for pre-concentration trace amounts of aflatoxins, the amount of Aflatoxins B1 and B2 in powdered milk was determined. Determination of aflatoxins was done by using high-performance liquid chromatography coupled with fluorescent detector. Method: Samples were extracted by immunoaffinity column (IAC) clean-up, and the...
متن کاملSeparation of aflatoxins by column chromatography.
Several column chromatographic methods are available for the separation of aflatoxins (l-6). Most of these procedures are elaborate and involve several steps of purification. A rapid and simple method of obtaining pure aflatoxins was reported by Steyn (5); however, with this method, we were not able to obtain pure crystalline aflatoxins B, and G1 for our experimental studies on animals. When pu...
متن کاملReverse phase analytical and preparative high pressure liquid chromatography of aflatoxins.
Reverse phase high pressure liquid chroma· k)graphy (HPLC) was investigated and found applicahle for determining 6 aflatoxins. Afla. toxins Mz• Mt• Gz• Gt , and Bt were completely resolved and Bz was satisfactorily separated on aCtS (10 ,urn. 4 mm id X 30 em) column with an eluting solvent of acetonitrile.water (35+ 65) and flow rate of 1.5 ml/min. Compounds were detected by ultraviolet absorba...
متن کاملOccurrence of Aflatoxins in Peanuts and Peanut Products Determined by Liquid Chromatography with Fluorescence Detection
Liquid chromatography with fluorescence detection using immunoaffinity column clean-up was a method described for determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2) in peanuts and peanut based products. The validation of the procedure was performed. Good coefficient of correlation was found for all aflatoxins in the range of 0.9993–0.9999. Limit of detection (LOD) and limit of quantificati...
متن کاملCleanup procedure for determination of aflatoxins in major agricultural commodities by liquid chromatography.
A simple, fast, reliable, and inexpensive chemical cleanup procedure was developed for quantitation of aflatoxins in major important agricultural commodities by liquid chromatography (LC). Aflatoxins were extracted from a ground sample with methanol-water (80 + 20, v/v), and after a single cleanup step on a minicolumn packed with basic aluminum oxide, they were quantitated by LC equipped with a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1971
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.35.417